Fig 2: The surface topography analyzed by tapping mode AFM. (a) Bare Si3N4 surface, (b) RCA-1-treated Si3N4 surface, (c) APTES-modified Si3N4 surface, (d) APTES-GA-modified surface, (e) 20 µg/mL HLA-A-immobilized surface and (f) 20 µg/mL MICA*010-immobilized surface.

Fig 3: Integrated fluorescence density of FITC-tagged antibodies bound on the silicon surface after immunoassay. (a,d) the fluorescent signal captured from the silicon wafer probed with IgG1, ?appa isotype antibody, (b) the fluorescent signal captured from anti-HLA probing surface, (c) integrated densities of immobilized HLA-A surface probed with anti-HLA-A, (e) the fluorescent signal captured from the anti-MICA probing surface, (f) integrated densities of immobilized MICA*010 surface probed with anti-MICA.